Pjet.

pJET.2/blunt, the positive-selection vector. Steps 1. Set up the ligation reaction on ice: Component Volume 2X reaction buffer 10 µL Unpurified PCR product, purified PCR product, or other blunt-end DNA fragment 1 µL (0.15 pmol ends) pJET1.2/blunt (50 ng/µL) 1 µL (0.05 pmol ends) Water, nuclease-free To 19 µL T4 DNA ligase 1 µL Total ...PCR products were then cloned into pJET1.2/blunt (Thermo Fisher Scientific). The resultant plasmid pJET-nprA was digested with NsiI and then self-ligated to generate the repair template pJET-∆nprA. Similarly, pJET-aprA was digested with BstBI and SacI, treated with T4 DNA polymerase for end-blunting, and self-ligated to generate …WebBecause pJET is ~10× longer than the backbone, a threshold of at least 5 repeats was applied during consensus calling. Even so, only 7.8–10.9% of these reads contained enough copies of the ...WebSpecial Features. Self Sealing Fuel Tanks, Radio Navigation II, Air-Ground Radar II, Bomb Sights I, 2 Armor Plates. Keep in mind that these designs can still be modified to suit your individual needs, like adding more range or reducing fuel costs. These plane designs should help you control the skies from the beginning of your campaign to …Web

pJET Cloning. Followed Thermo Scientific CloneJET PCR Blunt-End Cloning Protocol. 1. Combine the following reagents sequentially on ice. A total volume of 18 ...PJET is the most innovative, easy to use, hygienic & effective Portable Jet Spray in the market. CUSTOM PRESSURE CONTROL :- The water pressure can be easity controlled by its special feature of Soft Spray & Hard Spray, so that it can provide you with a convenient and easy way to maintain your personal hygiene. also it has a 180 degrees ...Web

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SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of your plasmids, DNA …

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Find & Download Free Graphic Resources for Black Png. 97,000+ Vectors, Stock Photos & PSD files. Free for commercial use High Quality ImagesThe mcr-1-bearing recombinant plasmid with the tetracycline resistant gene (tetA) designated as pJET-TC-MCR-1 (7,489 bps) was generated. We then transformed the pJET-TC-MCR-1 and pJET-TC (control) plasmids into the mcr-1 plasmid-cured strain PC-KP2509, and compared their stabilities in the common host. The transformants were passaged daily ...

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This pJET 1.2 vector has a T7 promoter region so in theory should it be able to express a functional insert? View. How much DNA template (genomic or plasmid DNA) is used for a general PCR?

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